In vitro safety & efficacy Evaluation


BioSpectrum Life Science Institute (BLSI)


Key activity

· Skin cell based screening system.
· Assess mechanisms of action and safety at material discovery.
· Support for material development work.



In vitro safety & efficacy Evaluation

Category Efficacy service Test Period
Skin irritation
(Erythema, inflammation)
- Cytotoxicity assay
- Antioxidant test (DPPH, SOD, Lipid peroxidation assay, Nitric oxide, ROS)
- Inflammatory cytokine assay (TNF-α, IL-8, COX-2, Eotaxin-1)
4-6 weeks after the products reception
Pigmentation disorder
(Melasma, hyperpigmentation, vitiligo)
- Mushroom tyrosinase activity assay
- Cellular tyrosinase activity assay
- Melanin contents assay
4-6 weeks after the products reception
Skin-ageing
(Wrinkle, Stem cell, photoageing)
- Cell proliferation (skin self-renewal)
- ECM production (collagen, elastin, Hyaluronic acid)
- DNA damage assay (UV radiation and Oxidative stress)
- AGEs production assay
- Cellular senescence assay (SA-beta-Gal assay)
4-6 weeks after the products reception
Inflammatory skin
(Atopy, psoriasis, acne, seborrhea dermatitis)
- Immune regulation : IL-2
- Inflammatory cytokine assay (TNF-α, IL-1, IL-4, IL-8, COX-2, Eotaxin-1)
- NF-κB luciferase activity assay
4-6 weeks after the products reception
Infection
(Acne, dandruff, athletes foot, atopy)
- Disk diffusion test(P.acne, P.ovale, S.aureus, P.aeruginosa, E.coli, C.albican, etc,)
- MIC test(Determination of minimal inhibition concentration)
- Challenge Test(Gram +/Gram -, Yeast, Fungi)
4-8 weeks after the products reception
Obesity
(Sliming)
-oil-red O staining/Tri-glyceride assay
-Lipolysis(Glycerol release assay)
-GPDH activity inhibition assay
4-8 weeks after the products reception
Hair - Hair follicle dermal papilla cell proliferation
- Keratinocytes proliferation
4-8 weeks after the products reception
Toxicology test - Phototoxicity test(3T3NRU phototoxicity test)
- Eye irritation (HET-CAM)
4-6 weeks after the products reception